Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila.
Identifieur interne : 000051 ( Main/Exploration ); précédent : 000050; suivant : 000052Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila.
Auteurs : P Githure M'Angale [Canada] ; Brian E. Staveley [Canada]Source :
- Genome [ 1480-3321 ] ; 2017.
English descriptors
- KwdEn :
- Age of Onset, Animals, Cell Survival, Culture Media, Drosophila Proteins (genetics), Drosophila melanogaster (genetics), Gene Expression Regulation, Genes, Recessive, Genotype, Male, Microscopy, Electron, Scanning, Mitochondria, Movement, Neurons (metabolism), Parkinson Disease (genetics), Phenotype, Photoreceptor Cells, Invertebrate, Proportional Hazards Models, Protein-Serine-Threonine Kinases (genetics), Proto-Oncogene Proteins c-bcl-2 (genetics), RNA Interference, Temperature, Transgenes, Ubiquitin-Protein Ligases (genetics).
- MESH :
- chemical , genetics : Drosophila Proteins, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins c-bcl-2, Ubiquitin-Protein Ligases.
- chemical : Culture Media.
- genetics : Drosophila melanogaster, Parkinson Disease.
- metabolism : Neurons.
- Age of Onset, Animals, Cell Survival, Gene Expression Regulation, Genes, Recessive, Genotype, Male, Microscopy, Electron, Scanning, Mitochondria, Movement, Phenotype, Photoreceptor Cells, Invertebrate, Proportional Hazards Models, RNA Interference, Temperature, Transgenes.
Abstract
Mutations in parkin (PARK2) and Pink1 (PARK6) are responsible for autosomal recessive forms of early onset Parkinson's disease (PD). Attributed to the failure of neurons to clear dysfunctional mitochondria, loss of gene expression leads to loss of nigrostriatal neurons. The Pink1/parkin pathway plays a role in the quality control mechanism aimed at eliminating defective mitochondria, and the failure of this mechanism results in a reduced lifespan and impaired locomotor ability, among other phenotypes. Inhibition of parkin or Pink1 through the induction of stable RNAi transgene in the Ddc-Gal4-expressing neurons results in such phenotypes to model PD. To further evaluate the effects of the overexpression of the Bcl-2 homologue Buffy, we analysed lifespan and climbing ability in both parkin-RNAi- and Pink1-RNAi-expressing flies. In addition, the effect of Buffy overexpression upon parkin-induced developmental eye defects was examined through GMR-Gal4-dependent expression. Curiously, Buffy overexpression produced very different effects: the parkin-induced phenotypes were enhanced, whereas the Pink1-enhanced phenotypes were suppressed. Interestingly, the overexpression of Buffy along with the inhibition of parkin in the neuron-rich eye results in the suppression of the developmental eye defects.
DOI: 10.1139/gen-2016-0165
PubMed: 28106473
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila.</title><author><name sortKey="M Angale, P Githure" sort="M Angale, P Githure" uniqKey="M Angale P" first="P Githure" last="M'Angale">P Githure M'Angale</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9</wicri:regionArea><wicri:noRegion>NL A1B 3X9</wicri:noRegion></affiliation></author><author><name sortKey="Staveley, Brian E" sort="Staveley, Brian E" uniqKey="Staveley B" first="Brian E" last="Staveley">Brian E. Staveley</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9</wicri:regionArea><wicri:noRegion>NL A1B 3X9</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28106473</idno><idno type="pmid">28106473</idno><idno type="doi">10.1139/gen-2016-0165</idno><idno type="wicri:Area/PubMed/Corpus">000046</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000046</idno><idno type="wicri:Area/PubMed/Curation">000046</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000046</idno><idno type="wicri:Area/PubMed/Checkpoint">000046</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000046</idno><idno type="wicri:Area/Ncbi/Merge">002193</idno><idno type="wicri:Area/Ncbi/Curation">002193</idno><idno type="wicri:Area/Ncbi/Checkpoint">002193</idno><idno type="wicri:Area/Main/Merge">000051</idno><idno type="wicri:Area/Main/Curation">000051</idno><idno type="wicri:Area/Main/Exploration">000051</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Overexpression of Buffy enhances the loss of parkin and suppresses the loss of Pink1 phenotypes in Drosophila.</title><author><name sortKey="M Angale, P Githure" sort="M Angale, P Githure" uniqKey="M Angale P" first="P Githure" last="M'Angale">P Githure M'Angale</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9</wicri:regionArea><wicri:noRegion>NL A1B 3X9</wicri:noRegion></affiliation></author><author><name sortKey="Staveley, Brian E" sort="Staveley, Brian E" uniqKey="Staveley B" first="Brian E" last="Staveley">Brian E. Staveley</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, Memorial University of Newfoundland, St. John's, NL A1B 3X9</wicri:regionArea><wicri:noRegion>NL A1B 3X9</wicri:noRegion></affiliation></author></analytic><series><title level="j">Genome</title><idno type="eISSN">1480-3321</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Age of Onset</term><term>Animals</term><term>Cell Survival</term><term>Culture Media</term><term>Drosophila Proteins (genetics)</term><term>Drosophila melanogaster (genetics)</term><term>Gene Expression Regulation</term><term>Genes, Recessive</term><term>Genotype</term><term>Male</term><term>Microscopy, Electron, Scanning</term><term>Mitochondria</term><term>Movement</term><term>Neurons (metabolism)</term><term>Parkinson Disease (genetics)</term><term>Phenotype</term><term>Photoreceptor Cells, Invertebrate</term><term>Proportional Hazards Models</term><term>Protein-Serine-Threonine Kinases (genetics)</term><term>Proto-Oncogene Proteins c-bcl-2 (genetics)</term><term>RNA Interference</term><term>Temperature</term><term>Transgenes</term><term>Ubiquitin-Protein Ligases (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Drosophila Proteins</term><term>Protein-Serine-Threonine Kinases</term><term>Proto-Oncogene Proteins c-bcl-2</term><term>Ubiquitin-Protein Ligases</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drosophila melanogaster</term><term>Parkinson Disease</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Neurons</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Age of Onset</term><term>Animals</term><term>Cell Survival</term><term>Gene Expression Regulation</term><term>Genes, Recessive</term><term>Genotype</term><term>Male</term><term>Microscopy, Electron, Scanning</term><term>Mitochondria</term><term>Movement</term><term>Phenotype</term><term>Photoreceptor Cells, Invertebrate</term><term>Proportional Hazards Models</term><term>RNA Interference</term><term>Temperature</term><term>Transgenes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Mutations in parkin (PARK2) and Pink1 (PARK6) are responsible for autosomal recessive forms of early onset Parkinson's disease (PD). Attributed to the failure of neurons to clear dysfunctional mitochondria, loss of gene expression leads to loss of nigrostriatal neurons. The Pink1/parkin pathway plays a role in the quality control mechanism aimed at eliminating defective mitochondria, and the failure of this mechanism results in a reduced lifespan and impaired locomotor ability, among other phenotypes. Inhibition of parkin or Pink1 through the induction of stable RNAi transgene in the Ddc-Gal4-expressing neurons results in such phenotypes to model PD. To further evaluate the effects of the overexpression of the Bcl-2 homologue Buffy, we analysed lifespan and climbing ability in both parkin-RNAi- and Pink1-RNAi-expressing flies. In addition, the effect of Buffy overexpression upon parkin-induced developmental eye defects was examined through GMR-Gal4-dependent expression. Curiously, Buffy overexpression produced very different effects: the parkin-induced phenotypes were enhanced, whereas the Pink1-enhanced phenotypes were suppressed. Interestingly, the overexpression of Buffy along with the inhibition of parkin in the neuron-rich eye results in the suppression of the developmental eye defects.</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="M Angale, P Githure" sort="M Angale, P Githure" uniqKey="M Angale P" first="P Githure" last="M'Angale">P Githure M'Angale</name></noRegion><name sortKey="Staveley, Brian E" sort="Staveley, Brian E" uniqKey="Staveley B" first="Brian E" last="Staveley">Brian E. Staveley</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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